[1] What is the process of joining two DNA strands together called?

a) Replication

b) Transcription

c) Translation

d) Ligation

[2] What type of enzyme is used in the ligation process?

a) RNA polymerase

b) DNA polymerase

c) Ligase

d) Restriction enzyme

[3] What is the purpose of ligation in biotechnology?

a) To create a new DNA strand

b) To cut DNA strands

c) To join two DNA strands together

d) To modify existing DNA strands

[4] Which of the following is a common application of ligation in biotechnology?

a) Gene therapy

b) Genetic engineering

c) DNA fingerprinting

d) All of the above

[5] What is the name of the enzyme that joins two DNA strands together during ligation?

a) RNA polymerase

b) DNA polymerase

c) Ligase

d) Restriction enzyme

[6] What is the process called when a restriction enzyme is used to cut a DNA strand and then ligation is used to join it to another DNA strand?

a) Cloning

b) Transcription

c) Translation

d) Replication

[7] Which of the following is not a step in the ligation process?

a) Cutting DNA strands with a restriction enzyme

b) Joining DNA strands with ligase

c) Replicating DNA strands

d) Purifying the ligated DNA

[8] How does ligation differ from PCR?

a) Ligation is used to join DNA strands together, while PCR is used to amplify DNA strands

b) Ligation is used to cut DNA strands, while PCR is used to amplify DNA strands

c) Ligation is used to purify DNA strands, while PCR is used to amplify DNA strands

d) Ligation is used to replicate DNA strands, while PCR is used to amplify DNA strands

[9] How does ligation differ from DNA sequencing?

a) Ligation is used to join DNA strands together, while DNA sequencing is used to determine the order of nucleotides in a DNA strand

b) Ligation is used to cut DNA strands, while DNA sequencing is used to determine the order of nucleotides in a DNA strand

c) Ligation is used to purify DNA strands, while DNA sequencing is used to determine the order of nucleotides in a DNA strand

d) Ligation is used to replicate DNA strands, while DNA sequencing is used to determine the order of nucleotides in a DNA strand

[10] What is the name of the enzyme that joins two DNA strands together during ligation?

a) RNA polymerase

b) DNA polymerase

c) Ligase

d) Restriction enzyme

Answers:

1. d) Ligation - The process of joining two DNA strands together is called ligation.
2. c) Ligase - The enzyme used in the ligation process is called ligase.
3. c) To join two DNA strands together - The purpose of ligation in biotechnology is to join two DNA strands together, allowing for the creation of new genetic combinations or the modification of existing DNA.
4. d) All of the above - Common applications of ligation in biotechnology include gene therapy, genetic engineering, and DNA fingerprinting.
5. c) Ligase - The enzyme that joins two DNA strands together during ligation is called ligase.
6. a) Cloning - The process of using a restriction enzyme to cut a DNA strand and then ligating it to another DNA strand is called cloning.
7. c) Replicating DNA strands - Replicating DNA strands is not a step in the ligation process, but rather a separate process used for creating multiple copies of a DNA strand.
8. a) Ligation is used to join DNA strands together, while PCR is used to amplify DNA strands - Ligation is used to join two DNA strands together, while PCR (polymerase chain reaction) is used to make multiple copies of a specific DNA strand.
9. a) Ligation is used to join DNA strands together, while DNA sequencing is used to determine the order of nucleotides in a DNA strand - Ligation is used to join two DNA strands together, while DNA sequencing is used to determine the specific sequence of nucleotides in a DNA strand.
10. c) Ligase - The enzyme that joins two DNA strands together during ligation is called ligase.