Whatsapp

[1] Which of the following is not a method of DNA sequencing?

A. Sanger sequencing

B. Next-generation sequencing

C. Gel electrophoresis

D. PCR

[2] In Sanger sequencing, what is the role of dideoxynucleotides?

A. To amplify the DNA sample

B. To stop DNA synthesis

C. To separate DNA fragments

D. To add fluorescent labels

[3] Which of the following is a disadvantage of Sanger sequencing?

A. It is time-consuming

B. It is expensive

C. It is not suitable for long DNA sequences

D. All of the above

[4] Next-generation sequencing uses which technology to read DNA sequences?

A. Gel electrophoresis

B. PCR

C. Microarrays

D. None of the above

[5] Which of the following is a benefit of next-generation sequencing?

A. It is faster and cheaper than Sanger sequencing

B. It can sequence long DNA sequences

C. It can sequence multiple samples at once

D. All of the above

[6] Which of the following is a disadvantage of next-generation sequencing?

A. It is more accurate than Sanger sequencing

B. It can only sequence small DNA fragments

C. It has lower resolution than Sanger sequencing

D. None of the above

[7] In DNA sequencing, what is the role of primer?

A. To start DNA synthesis

B. To stop DNA synthesis

C. To amplify the DNA sample

D. To separate DNA fragments

[8] What is the role of sequencing software in DNA sequencing?

A. To analyze the sequencing data

B. To generate the sequencing primer

C. To separate DNA fragments

D. To amplify the DNA sample

[9] Which of the following is not a type of next-generation sequencing?

A. Illumina sequencing

B. PacBio sequencing

C. Gel electrophoresis sequencing

D. Oxford Nanopore sequencing

[10] In DNA sequencing, what is the role of base-calling?

A. To determine the sequence of nucleotides

B. To amplify the DNA sample

C. To separate DNA fragments

D. To add fluorescent labels

Answers:

1.     C
2.     B
3.     D
4.     D
5.     D
6.     A
7.     A
8.     A
9.     C
10.     A

Reason:

1.     Gel electrophoresis is not a method of DNA sequencing, it is used to separate DNA fragments.
2.     Dideoxynucleotides are used to stop DNA synthesis in Sanger sequencing, allowing for the separation of DNA fragments based on size.
3.     Sanger sequencing is time-consuming, expensive, and not suitable for long DNA sequences.
4.     Next-generation sequencing uses various technologies such as Illumina, PacBio, and Oxford Nanopore to read DNA sequences.
5.     Next-generation sequencing is faster, cheaper, and can sequence long DNA sequences and multiple samples at once.
6.     Next-generation sequencing is not more accurate than Sanger sequencing and can only sequence small DNA fragments.
7.     Primers are used to start DNA synthesis in DNA sequencing.
8.     Sequencing software is used to analyze the sequencing data.
9.     Gel electrophoresis sequencing is not a type of next-generation sequencing.
10.     Base-calling is used to determine the sequence of nucleotides in DNA sequencing.